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mU6-sgRNA-CLTA-mCherry
(Plasmid #240637)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 240637 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pSICO derivative
  • Backbone size w/o insert (bp) 3996
  • Total vector size (bp) 5499
  • Vector type
    Mammalian Expression, CRISPR
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    puro-T2A-mCherry
  • gRNA/shRNA sequence
    CTGTGGTGCCGACTGGGAGC
  • Species
    H. sapiens (human)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mU6-sgRNA-CLTA-mCherry was a gift from James Nuñez (Addgene plasmid # 240637 ; http://n2t.net/addgene:240637 ; RRID:Addgene_240637)

  • For your References section:

    Programmable epigenome editing by transient delivery of CRISPR epigenome editor ribonucleoproteins. Xu D, Besselink S, Ramadoss GN, Dierks PH, Lubin JP, Pattali RK, Brim JI, Christenson AE, Colias PJ, Ornelas IJ, Nguyen CD, Chasins SE, Conklin BR, Nunez JK. bioRxiv [Preprint]. 2024 Nov 28:2024.11.26.625496. doi: 10.1101/2024.11.26.625496. 10.1101/2024.11.26.625496 PubMed 39651312
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