pCR175
(Plasmid #240640)

• Purpose: Template for in vitro transcription of mTurquoise2 mRNA using SP6 polymerase.
• Depositing Lab: John Pringle
• Publication: Aiptasia Genetic Tools Plasmids (unpublished)

Backbone

• Vector backbone: pCS107
• Backbone manufacturer: Baker et al., 1999
• Backbone size w/o insert (bp): 4108
• Total vector size (bp): 4834
• Vector type: Vertebrate gene expression and general in vitro transcription.

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mTurquoise2
• Species: Synthetic
• Insert Size (bp): 719
• GenBank ID: KM670033.1
• Promoter: SP6

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: Pcmv_end_seq_fwd (GCAAATGGGCGGTAGGCG)
• 3′ sequencing primer: M13 Reverse (-27) (CAGGAAACAGCTATGAC)

Resource Information

• Supplemental Documents:
  • pCR175.gb
• A portion of this plasmid was derived from a plasmid made by: pCS107 backbone: Baker JC, Beddington RSP, Harland RM. 1999. Wnt signaling in Xenopus embryos inhibits Bmp4 expression and activates neural development. Genes Dev. 13(23):3149–3159. doi:10.1101/gad.13.23.3149. mTurquoise2: Goedhart J, Von Stetten D, Noirclerc-Savoye M, Lelimousin M, Joosen L, Hink MA, Van Weeren L, Gadella TWJ, Royant A. 2012. Structure-guided evolution of cyan fluorescent proteins towards a quantum yield of 93%. Nat Commun. 3(1):751. doi:10.1038/ncomms1738. Plasmid mScarlet-I-mTurquoise2 (Addgene plasmid # 98839): Mastop M, Bindels DS, Shaner NC, Postma M, Gadella TWJ, Goedhart J. 2017. Characterization of a spectrally diverse set of fluorescent proteins as FRET acceptors for mTurquoise2. Sci Rep. 7(1):11999. doi:10.1038/s41598-017-12212-x.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry