pCR178
(Plasmid #240641)

• Purpose: Template for in vitro transcription of mScarlet-I mRNA using SP6 polymerase.
• Depositing Lab: John Pringle
• Publication: Aiptasia Genetic Tools Plasmids (unpublished)

Backbone

• Vector backbone: pCS107
• Backbone manufacturer: Baker et al., 1999
• Backbone size w/o insert (bp): 4108
• Total vector size (bp): 4813
• Vector type: Vertebrate gene expression and general in vitro transcription

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mScarlet-I
• Species: Synthetic
• Insert Size (bp): 699
• GenBank ID: KY021424.1
• Promoter: SP6

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: Pcmv_end_seq_fwd (GCAAATGGGCGGTAGGCG)
• 3′ sequencing primer: M13 Reverse (-27) (CAGGAAACAGCTATGAC)

Resource Information

• Supplemental Documents:
  • pCR178.gb
• A portion of this plasmid was derived from a plasmid made by: pCS107 backbone: Baker JC, Beddington RSP, Harland RM. 1999. Wnt signaling in Xenopus embryos inhibits Bmp4 expression and activates neural development. Genes Dev. 13(23):3149–3159. doi:10.1101/gad.13.23.3149. mScarlet-I: Bindels DS, Haarbosch L, Van Weeren L, Postma M, Wiese KE, Mastop M, Aumonier S, Gotthard G, Royant A, Hink MA, et al. 2017. mScarlet: a bright monomeric red fluorescent protein for cellular imaging. Nat Methods. 14(1):53–56. doi:10.1038/nmeth.4074. Plasmid mScarlet-I-mTurquoise2 (Addgene plasmid # 98839): Mastop M, Bindels DS, Shaner NC, Postma M, Gadella TWJ, Goedhart J. 2017. Characterization of a spectrally diverse set of fluorescent proteins as FRET acceptors for mTurquoise2. Sci Rep. 7(1):11999. doi:10.1038/s41598-017-12212-x.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry