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pREC1010-Abe1
(Plasmid #240683)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 240683 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pORTMAGE-Ec1
  • Backbone manufacturer
    George Church (Addgene #138474)
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Abe1 RT, Abe1 ncRNA, CspRecT and EcSSB
  • Species
    Synthetic
  • Insert Size (bp)
    2670
  • Mutation
    WT

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2025.06.16.660010 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pREC1010-Abe1 was a gift from Seth Shipman (Addgene plasmid # 240683 ; http://n2t.net/addgene:240683 ; RRID:Addgene_240683)

  • For your References section:

    Genome editing of phylogenetically distinct bacteria using portable retron-mediated recombineering. González-Delgado A, Bonillo-Lopez L, Johnson MS, Knödlseder N, Ko CC, Lekbach Y, Oh JH, Selvakumar H, Wold MC, Yu Z, Aragón V, Gralnick JA, Güell M, Hatfull GF, Keitz BK, Koskella B, Mutalik VK, van Pijkeren JP, Shipman SL. bioRxiv 2025.06.16.660010 10.1101/2025.06.16.660010
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