pREC1010lac-Ebu1
(Plasmid
#240699)
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PurposeRSF1010 origin of replication plasmid containing Ebu1 recombitron with a SapI flanked stuffer in the ncRNA expressed by lac promoter
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 240699 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 |
Backbone
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Vector backbonepORTMAGE-Ec1
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Backbone manufacturerGeorge Church (Addgene #138474)
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameEbu1 RT, Ebu1 ncRNA, CspRecT and EcSSB
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SpeciesSynthetic
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Insert Size (bp)2710
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MutationWT
Cloning Information
- Cloning method Gibson Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pREC1010lac-Ebu1 was a gift from Seth Shipman (Addgene plasmid # 240699 ; http://n2t.net/addgene:240699 ; RRID:Addgene_240699)