pRECBRR1-Kva1
(Plasmid
#240703)
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Purposeinverted BRR1 origin of replication plasmid containing Kva1 recombitron with a donor in the ncRNA to target phzM locus in Pseudomonas aeruginosa PAO1 expressed by Pm promoter
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 240703 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 |
Backbone
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Vector backbonepORTMAGE-Pa1
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Backbone manufacturerGeorge Church (Addgene #138474)
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Gentamicin, 10 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameKva1 RT,Kva1 ncRNA, PaRecT and PaSSB
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SpeciesSynthetic
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Insert Size (bp)2598
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MutationInverted BRR1 origin of replication
Cloning Information
- Cloning method Gibson Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRECBRR1-Kva1 was a gift from Seth Shipman (Addgene plasmid # 240703 ; http://n2t.net/addgene:240703 ; RRID:Addgene_240703)