BCTVΔREP-YPET-pDGB3alpha1
(Plasmid
#240725)
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PurposeBCTV vector for YPET expression. Rep has been mutated.
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 240725 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepDGB3alpha1
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Vector typePlant Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameYPET
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SpeciesBeet Curly Top Virus
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Mutation2 stop codons inserted in Rep
Cloning Information
- Cloning method Golden Gate
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
BCTVΔREP-YPET-pDGB3alpha1 was a gift from Jose Alonso (Addgene plasmid # 240725 ; http://n2t.net/addgene:240725 ; RRID:Addgene_240725) -
For your References section:
Development of Modular Geminivirus-Based Vectors for High Cargo Expression and Gene Targeting in Plants. Neubauer M, Vollen K, Zhao C, Ascencio-Ibanez JT, Hanley-Bowdoin L, Stepanova AN, Alonso JM. Plant Biotechnol J. 2025 Sep 21. doi: 10.1111/pbi.70320. 10.1111/pbi.70320 PubMed 40976866