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Addgene

Sc mec1-S6-P/FLAG-pRS403/GAL-L
(Plasmid #240737)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 240737 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pRS403/GAL
  • Vector type
    Yeast Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mec1-S6-P/FLAG
  • Species
    S. cerevisiae (budding yeast)
  • Entrez Gene
    MEC1 (a.k.a. YBR136W, ESR1, RAD31, SAD3)
  • Tag / Fusion Protein
    • S6, followed by PreScission Protease site, followed by 3X FLAG (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Sc mec1-S6-P/FLAG-pRS403/GAL-L was a gift from Michael O'Donnell (Addgene plasmid # 240737 ; http://n2t.net/addgene:240737 ; RRID:Addgene_240737)
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