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Addgene

Sc RFA[1+2]-pET
(Plasmid #240742)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 240742 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pET(11a)
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert 1

  • Gene/Insert name
    RFA1
  • Species
    S. cerevisiae (budding yeast)
  • Entrez Gene
    RFA1 (a.k.a. YAR007C, BUF2, FUN3, RPA1, RPA70)

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)
  • 5′ sequencing primer Unknown
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    RFA2
  • Species
    S. cerevisiae (budding yeast)
  • Entrez Gene
    RFA2 (a.k.a. YNL312W, BUF1, RPA2, RPA32)

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)
  • 5′ sequencing primer Unknown
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Sc RFA[1+2]-pET was a gift from Michael O'Donnell (Addgene plasmid # 240742 ; http://n2t.net/addgene:240742 ; RRID:Addgene_240742)
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