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Addgene

SAGA002
(Plasmid #240751)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 240751 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pSEVAx21
  • Vector type
    Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Tetracycline, 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    SAGA RK2 J23100 BCD_medium - gfp - tetARK2- CmTrunc
  • Species
    Unspecified
  • Mutation
    Mutation on translation initiation region of tetA

Cloning Information

  • Cloning method Other

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    SAGA002 was a gift from Morten Norholm (Addgene plasmid # 240751 ; http://n2t.net/addgene:240751 ; RRID:Addgene_240751)

  • For your References section:

    Simple and robust in vivo engineering of plasmid DNA at any copy number in Escherichia coli. Sepulchro AGV, Kozlowski HCJ, Norholm MHH. Commun Biol. 2025 Jun 17;8(1):933. doi: 10.1038/s42003-025-08361-9. 10.1038/s42003-025-08361-9 PubMed 40527904
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