SAGA039
(Plasmid
#240788)
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PurposePlasmid contains the triple selection cassette gfp -RK2 tetARK2 - CmTrunc that allows for in vivo recombineering in E.coli.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 240788 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 |
Backbone
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Vector backbonepSEVAx21
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Vector typeSynthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Tetracycline, 10 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameSAGA RK2 His low : T7- His tags low - gfp -RK2 tetARK2 - CmTrunc
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SpeciesUnspecified
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MutationMutation on translation initiation region of tetA
Cloning Information
- Cloning method Other
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
SAGA039 was a gift from Morten Norholm (Addgene plasmid # 240788 ; http://n2t.net/addgene:240788 ; RRID:Addgene_240788) -
For your References section:
Simple and robust in vivo engineering of plasmid DNA at any copy number in Escherichia coli. Sepulchro AGV, Kozlowski HCJ, Norholm MHH. Commun Biol. 2025 Jun 17;8(1):933. doi: 10.1038/s42003-025-08361-9. 10.1038/s42003-025-08361-9 PubMed 40527904