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Addgene

SAGA039
(Plasmid #240788)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 240788 Standard format: Plasmid sent in bacteria as agar stab 1 $89

Backbone

  • Vector backbone
    pSEVAx21
  • Vector type
    Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Tetracycline, 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    SAGA RK2 His low : T7- His tags low - gfp -RK2 tetARK2 - CmTrunc
  • Species
    Unspecified
  • Mutation
    Mutation on translation initiation region of tetA

Cloning Information

  • Cloning method Other

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    SAGA039 was a gift from Morten Norholm (Addgene plasmid # 240788 ; http://n2t.net/addgene:240788 ; RRID:Addgene_240788)
  • For your References section:

    Simple and robust in vivo engineering of plasmid DNA at any copy number in Escherichia coli. Sepulchro AGV, Kozlowski HCJ, Norholm MHH. Commun Biol. 2025 Jun 17;8(1):933. doi: 10.1038/s42003-025-08361-9. 10.1038/s42003-025-08361-9 PubMed 40527904