SAGA042
(Plasmid
#240791)
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PurposePlasmid contains the triple selection cassette gfp -p15a tetAp15a - CmTrunc that allows for in vivo recombineering in E.coli.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 240791 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 |
Backbone
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Vector backbonepSEVAx61
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Vector typeSynthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Tetracycline, 10 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameSAGA p15a His low : T7- His tags low - gfp -p15a tetAp15a - CmTrunc
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SpeciesUnspecified
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MutationMutation on translation initiation region of tetA
Cloning Information
- Cloning method Other
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
SAGA042 was a gift from Morten Norholm (Addgene plasmid # 240791 ; http://n2t.net/addgene:240791 ; RRID:Addgene_240791) -
For your References section:
Simple and robust in vivo engineering of plasmid DNA at any copy number in Escherichia coli. Sepulchro AGV, Kozlowski HCJ, Norholm MHH. Commun Biol. 2025 Jun 17;8(1):933. doi: 10.1038/s42003-025-08361-9. 10.1038/s42003-025-08361-9 PubMed 40527904