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pFA6a-Pho8∆60-BleMX6
(Plasmid #240974)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 240974 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pFA6a
  • Total vector size (bp) 5254
  • Vector type
    Bacterial Expression
  • Selectable markers
    Zeocin ; Bleomycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    BleMX6
  • gRNA/shRNA sequence
    gRNA
  • Species
    S. cerevisiae (budding yeast)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Asc1 (not destroyed)
  • 3′ cloning site EcoRV (not destroyed)
  • 5′ sequencing primer CGCGCCACTTCTAAATA
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFA6a-Pho8∆60-BleMX6 was a gift from Fulvio Reggiori (Addgene plasmid # 240974 ; http://n2t.net/addgene:240974 ; RRID:Addgene_240974)

  • For your References section:

    A plasmid module for PCR-based gene modification for the accurate measurement of vacuolar delivery of specific proteins in yeast Saccharomyces cerevisiae. Valdbjorn Kanne J, Reggiori F. Autophagy Rep. 2025 May 31;4(1):2511724. doi: 10.1080/27694127.2025.2511724. eCollection 2025. 10.1080/27694127.2025.2511724 PubMed 40458443
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