pVH4p_3BNC117_muSplice
(Plasmid #241004)

• Purpose: For targeted gene knock-in at NHP IGH locus and B cell-specific expression of full-length 3BNC117 following endogenous splicing
• Depositing Lab: Jennifer Adair
• Publication: Castelli et al Mol Ther. 2026 Feb 2:S1525-0016(26)00081-X. doi: 10.1016/j.ymthe.2026.01.038.

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Backbone

• Vector backbone: pAAV-GFP
• Backbone manufacturer: Addgene
• Backbone size w/o insert (bp): 3925
• Total vector size (bp): 6661
• Vector type: Mammalian Expression, AAV, CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: 3BNC117
• Species: H. sapiens (human)
• Insert Size (bp): 2736
• Promoter: VH4

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: GATGGACATTCTGCCATTGTGAT
• 3′ sequencing primer: CTGTGCTAGTATTTCTTAGCTAA

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://doi.org/10.1101/2025.05.02.651933 for bioRxiv preprint.