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pTT3_3BNC117_NHP_IgG
(Plasmid #241008)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 241008 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pTT3
  • Backbone manufacturer
    Addgene
  • Backbone size w/o insert (bp) 5934
  • Total vector size (bp) 8264
  • Vector type
    Mammalian Expression, Affinity Reagent/ Antibody

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    3BNC117
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2328
  • Promoter CMV
  • Tag / Fusion Protein
    • 6x-His Avi-tag (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer GGAGCAGTGTTCGTCAGCCCTTCCGCTAGCGATATACAAATGACTCAAAG
  • 3′ sequencing primer AGGGAATACACTCGGGCCCTTGGTGCTAGCACTGCTAACAGTGACTTGGG
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2025.05.02.651933 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTT3_3BNC117_NHP_IgG was a gift from Jennifer Adair (Addgene plasmid # 241008 ; http://n2t.net/addgene:241008 ; RRID:Addgene_241008)

  • For your References section:

    In vivo production of an anti-HIV antibody in mice by non-viral gene knock-in in primate hematopoietic stem and progenitor cells. Castelli JMP, Poljakov K, Jwa Y, Cunningham R, Cassidy ME, Gray MD, Sanchez Gaytan JN, Enstrom MR, Gastelum G, Wang Z, Linton JD, Rongvaux A, Taylor JJ, Adair JE. Mol Ther. 2026 Feb 2:S1525-0016(26)00081-X. doi: 10.1016/j.ymthe.2026.01.038. 10.1016/j.ymthe.2026.01.038 PubMed 41635086
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