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pET-29_GSS-6xHis-SUMO-VidC-EGFP-FLAG
(Plasmid #241009)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 241009 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET-29
  • Backbone size w/o insert (bp) 5000
  • Total vector size (bp) 6747
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    GSS-6xHis-SUMO-VidC-EGFP-FLAG
  • Species
    Synthetic
  • Insert Size (bp)
    1473
  • Promoter T7
  • Tags / Fusion Proteins
    • His-tag (N terminal on insert)
    • SUMO-tag (N terminal on insert)
    • FLAG-tag (C terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET-29_GSS-6xHis-SUMO-VidC-EGFP-FLAG was a gift from Cole DeForest (Addgene plasmid # 241009 ; http://n2t.net/addgene:241009 ; RRID:Addgene_241009)

  • For your References section:

    One-Step Purification and N-Terminal Functionalization of Bioactive Proteins via Atypically Split Inteins. Gharios R, Li A, Kopyeva I, Francis RM, DeForest CA. Bioconjug Chem. 2024 Jun 19;35(6):750-757. doi: 10.1021/acs.bioconjchem.4c00223. Epub 2024 May 30. 10.1021/acs.bioconjchem.4c00223 PubMed 38815180
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