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pCMV-Frankenbody-HA-mScarlet3-IRESv4-HaloTag-tdMCP
(Plasmid #241135)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 241135 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pmCherry-N1
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    Anti-HA frankenbody
  • Species
    H. sapiens (human)
  • Promoter CMV
  • Tag / Fusion Protein
    • mScarlet3 (C terminal on insert)

Cloning Information for Gene/Insert 1

  • Cloning method Gibson Cloning

Gene/Insert 2

  • Gene/Insert name
    tdMCP
  • Tag / Fusion Protein
    • HaloTag (N terminal on insert)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV-Frankenbody-HA-mScarlet3-IRESv4-HaloTag-tdMCP was a gift from Tim Stasevich (Addgene plasmid # 241135 ; http://n2t.net/addgene:241135 ; RRID:Addgene_241135)

  • For your References section:

    All Probes Plasmids (APPs) for multicolor and long-term tracking of single-mRNA translation dynamics. Galindo G, Fixen GM, Heredia A, Morisaki T, Stasevich TJ. Mol Biol Cell. 2025 Jun 1;36(6):mr6. doi: 10.1091/mbc.E25-02-0091. 10.1091/mbc.E25-02-0091 PubMed 40366872
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