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pCRISPR-EASY
(Plasmid #241237)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 241237 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pcDNA3.1/pEF6-nls-YFP-2A-Cas9
  • Backbone size (bp) 11935
  • Modifications to backbone
    pEF6-nls-YFP-2A-Cas9 was digested with AatII and re-ligated. The cas9 gene was mutated to remove the two BsmBI recognition sequences but maintain the encoded protein sequence. The sgRNA scaffold from LentiCRISPRv2 (Addgene plasmid #52961) introduced at the AatII site.
  • Vector type
    Mammalian Expression, CRISPR
  • Promoter U6
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    This plasmid is a derivative of pEF6-nls-YFP-2A-Cas9 (Bowman et al., (2017) Cell Chem Biol, PMID 28479296)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCRISPR-EASY was a gift from Tamara O'Connor (Addgene plasmid # 241237 ; http://n2t.net/addgene:241237 ; RRID:Addgene_241237)

  • For your References section:

    An efficient and cost-effective method for disrupting genes in RAW264.7 macrophages using CRISPR-Cas9. Hossain MJ, O'Connor TJ. PLoS One. 2024 Mar 14;19(3):e0299513. doi: 10.1371/journal.pone.0299513. eCollection 2024. 10.1371/journal.pone.0299513 PubMed 38483963
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