GfaABC1D-DIO-Lck-mCherry-ibARK(D110A)-4x6T
(Plasmid
#241244)
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PurposeNegative control vrial expression vector for Cre-dependent expression plasma membrane-tethered ibARK with higher specificity in astrocytes
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 241244 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 |
Backbone
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Vector backboneUnknown
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Vector typeAAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameDIO-Lck-mCherry-ibARK(D110A)-4x6T
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SpeciesB. taurus (bovine)
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MutationD110A
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
GfaABC1D-DIO-Lck-mCherry-ibARK(D110A)-4x6T was a gift from Jun Nagai (Addgene plasmid # 241244 ; http://n2t.net/addgene:241244 ; RRID:Addgene_241244) -
For your References section:
The astrocytic ensemble acts as a multiday trace to stabilize memory. Dewa K, Kaseda K, Kuwahara A, Kubotera H, Yamasaki A, Awata N, Komori A, Holtz MA, Kasai A, Skibbe H, Takata N, Yokoyama T, Tsuda M, Numata G, Nakamura S, Takimoto E, Sakamoto M, Ito M, Masuda T, Nagai J. Nature (2025) 10.1038/s41586-025-09619-2