pmCh-N1 BLTP3A LIR mutant
(Plasmid
#241249)
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PurposeFor exogenous expression of codon optimized BLTP3A (UHRF1BP1) lacking its LIR motif as a C-terminal fusion to RFP in mammalian cells
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 241249 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepmCh-N1
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameBLTP3A (UHRF1BP1) Δ1129-1134
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SpeciesH. sapiens (human)
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MutationΔ1129-1134
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Entrez GeneBLTP3A (a.k.a. C6orf107, ICBP90, SHIP164B, UHRF1BP1, dJ349A12.1)
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Tag
/ Fusion Protein
- RFP (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pmCh-N1 BLTP3A LIR mutant was a gift from Pietro De Camilli (Addgene plasmid # 241249 ; http://n2t.net/addgene:241249 ; RRID:Addgene_241249) -
For your References section:
BLTP3A is associated with membranes of the late endocytic pathway and is an effector of CASM. Hanna MG, Rodriguez Cruz HO, Fujise K, Wu Y, Xu CS, Pang S, Li Z, Monetti M, De Camilli P. EMBO J (2025) 44: 6168 - 6195 10.1038/s44318-025-00543-9 PubMed 39386594