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pRSF:His6-CylA-27-412
(Plasmid #241275)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 241275 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pRSFDuet-1
  • Backbone size w/o insert (bp) 3829
  • Total vector size (bp) 4960
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CylA protease
  • Species
    Enterococcus faecalis
  • Insert Size (bp)
    1208
  • Mutation
    N-terminal secretion signal sequence removed (residues 1-27).
  • GenBank ID
    JQ794947.1
  • Promoter T7
  • Tag / Fusion Protein
    • 6xHis (N terminal on backbone)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer AAAAAGAATTCGCTGAGCAACAATATCAGCTTC
  • 3′ sequencing primer AAAAAGCGGCCGCTTATTTAATGCGCACTTCTTTGTA
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRSF:His6-CylA-27-412 was a gift from Wilfred van der Donk (Addgene plasmid # 241275 ; http://n2t.net/addgene:241275 ; RRID:Addgene_241275)
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