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pACT-HAB1_star
(Plasmid #241277)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 241277 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pACT
  • Vector type
    Yeast Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HAB1_star
  • Species
    A. thaliana (mustard weed)
  • Mutation
    NA
  • Entrez Gene
    HAB1 (a.k.a. AT1G72770, AtHAB1, F28P22.4, F28P22_4, HYPERSENSITIVE TO ABA1, homology to ABI1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Cutler lab uses this with yeast strain Mav99 but has tested it with Mav203 (commercially available) and it works well with that strain too. Please visit https://doi.org/10.1101/2025.05.15.654352 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pACT-HAB1_star was a gift from Sean Cutler (Addgene plasmid # 241277 ; http://n2t.net/addgene:241277 ; RRID:Addgene_241277)

  • For your References section:

    An orthogonalized PYR1-based CID module with reprogrammable ligand-binding specificity. Park SY, Qiu J, Wei S, Peterson FC, Beltran J, Medina-Cucurella AV, Vaidya AS, Xing Z, Volkman BF, Nusinow DA, Whitehead TA, Wheeldon I, Cutler SR. Nat Chem Biol. 2024 Jan;20(1):103-110. doi: 10.1038/s41589-023-01447-7. Epub 2023 Oct 23. 10.1038/s41589-023-01447-7 PubMed 37872402
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