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Addgene

pBD-PYR1
(Plasmid #241278)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 241278 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pBD-GAL4-cam
  • Vector type
    Yeast Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PYR1
  • Species
    A. thaliana (mustard weed)
  • Mutation
    NA
  • Entrez Gene
    PYR1 (a.k.a. AT4G17870, PYRABACTIN RESISTANCE 1, RCAR11, T6K21.50, T6K21_50, regulatory component of ABA receptor 11)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Cutler lab uses this with yeast strain Mav99 but has tested it with Mav203 (commercially available) and it works well with that strain too. Please visit https://doi.org/10.1101/2025.05.15.654352 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBD-PYR1 was a gift from Sean Cutler (Addgene plasmid # 241278 ; http://n2t.net/addgene:241278 ; RRID:Addgene_241278)

  • For your References section:

    Abscisic acid inhibits type 2C protein phosphatases via the PYR/PYL family of START proteins. Park SY, Fung P, Nishimura N, Jensen DR, Fujii H, Zhao Y, Lumba S, Santiago J, Rodrigues A, Chow TF, Alfred SE, Bonetta D, Finkelstein R, Provart NJ, Desveaux D, Rodriguez PL, McCourt P, Zhu JK, Schroeder JI, Volkman BF, Cutler SR. Science. 2009 May 22;324(5930):1068-71. doi: 10.1126/science.1173041. Epub 2009 Apr 30. 10.1126/science.1173041 PubMed 19407142
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