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Addgene

pBD_PYR1_156
(Plasmid #241285)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 241285 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pBD-GAL4-cam
  • Vector type
    Yeast Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Cutler lab uses this with yeast strain Mav99 but has tested it with Mav203 (commercially available) and it works well with that strain too. Please visit https://doi.org/10.1101/2025.05.15.654352 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBD_PYR1_156 was a gift from Sean Cutler (Addgene plasmid # 241285 ; http://n2t.net/addgene:241285 ; RRID:Addgene_241285)

  • For your References section:

    Unusually Broad-spectrum small-molecule sensing using a single protein scaffold. Tian H, Beltran J, George W, Lenert-Mondou C, Seder N, Davis ZI, Swift SD, Girke T, Whitehead TA, Wheeldon I, Cutler SR. bioRxiv [Preprint]. 2025 May 15:2025.05.15.654352. doi: 10.1101/2025.05.15.654352. 10.1101/2025.05.15.654352 PubMed 40462935
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