pBD_PYR1_156-F1
(Plasmid
#241286)
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Purpose(Empty Backbone) Backbone for PYR1 sensor library assembly from oligopools; stop codon removed; PYR1 sensor library assembly from oligopools;
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 241286 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 |
Backbone
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Vector backbonepBD-GAL4-cam
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Vector typeYeast Expression
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Cutler lab uses this with yeast strain Mav99 but has tested it with Mav203 (commercially available) and it works well with that strain too. Please visit https://doi.org/10.1101/2025.05.15.654352 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBD_PYR1_156-F1 was a gift from Sean Cutler (Addgene plasmid # 241286 ; http://n2t.net/addgene:241286 ; RRID:Addgene_241286)