mVenus-synaptojanin1(D730A,498-901)-CAAX
(Plasmid
#241304)
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PurposeMammalian expression of truncated form of 5-phosphatase-dead synaptojanin-1 with CAAX motif fused to mVenus
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 241304 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepEGFP-C1
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Backbone manufacturerClonetech
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSynaptojanin-1
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SpeciesH. sapiens (human)
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Insert Size (bp)1272
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Mutation498-901 aa, D730A
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Entrez GeneSYNJ1 (a.k.a. DEE53, EIEE53, INPP5G, PARK20)
- Promoter CMV
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Tag
/ Fusion Protein
- mVenus (N terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer CMV-F
- 3′ sequencing primer SV40-pArev
- (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byAddgene (#22293)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
mVenus-synaptojanin1(D730A,498-901)-CAAX was a gift from Justin Taraska (Addgene plasmid # 241304 ; http://n2t.net/addgene:241304 ; RRID:Addgene_241304) -
For your References section:
A PIP2-stabilized syntaxin-1a structure mapped with transition metal ion FRET and unnatural fluorescent amino acids at the plasma membrane. Obashi K, Strub MP, Taraska JW. Structure. 2025 Nov 24:S0969-2126(25)00388-0. doi: 10.1016/j.str.2025.10.001. 10.1016/j.str.2025.10.001 PubMed 41290003