pLV-U6-ZNF865-UbC-DsRed-P2A-Bsr
(Plasmid
#241309)
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PurposeLentiviral SpCas9-gRNA (ZNF865) expression vector with DsRed-Express2-P2A-BlastR
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 241309 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backboneFUGW
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Vector typeLentiviral
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Selectable markersdsRED
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameZNF865 gRNA
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gRNA/shRNA sequenceUnspecified
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SpeciesH. sapiens (human)
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Entrez GeneZNF865
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The BioRxiv pre-print is available at: https://www.biorxiv.org/content/10.1101/2025.06.13.659603v2
This plasmid was adapted from addgene #83919 with a ZNF865 gRNA clone in between esp3I sites.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLV-U6-ZNF865-UbC-DsRed-P2A-Bsr was a gift from Robby Bowles (Addgene plasmid # 241309 ; http://n2t.net/addgene:241309 ; RRID:Addgene_241309)