pLV-mU6-ACAN-hU6-Col2a1-hUbC-dsRed
(Plasmid
#241310)
-
PurposeLentiviral expression vector encoding for multiplex regulation of ACAN/Col2a1
-
Depositing Lab
-
Publication
-
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 241310 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
-
Vector backboneFUGW
-
Vector typeLentiviral
-
Selectable markersdsRED
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)NEB Stable
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameACAN/Col2a1 gRNA
-
gRNA/shRNA sequenceUnspecified
-
SpeciesH. sapiens (human)
-
Entrez GeneCOL2A1 (a.k.a. ANFH, AOM, COL11A3, SEDC, STL1)
Cloning Information
- Cloning method Golden Gate
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This plasmid was adapted from addgene #84034 before performing golden gate assembly to include ACAN and Col2a1 gRNA.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pLV-mU6-ACAN-hU6-Col2a1-hUbC-dsRed was a gift from Robby Bowles (Addgene plasmid # 241310 ; http://n2t.net/addgene:241310 ; RRID:Addgene_241310)