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pcDNA3.1-MSTN-D182N
(Plasmid #241323)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 241323 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pcDNA3.1 (+)
  • Backbone manufacturer
    Invitrogen (Life Technologies)
  • Backbone size w/o insert (bp) 5431
  • Total vector size (bp) 11679
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MSTN full gene
  • Alt name
    Myostatin
  • Species
    B. taurus (bovine)
  • Insert Size (bp)
    6248
  • Mutation
    p.D182N
  • GenBank ID
    GeneID:281187
  • Entrez Gene
    MSTN (a.k.a. GDF8)
  • Promoter CMV

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Addgene NGS finds six natural variants within MSTN intron 1 (NC_037329.1). The depositor confirms these intronic variants have no detectable effect on plasmid function, and that the plasmid has been tested and found fully functional in terms of RNA expression and splicing.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1-MSTN-D182N was a gift from Mathilde Dupont-Nivet (Addgene plasmid # 241323 ; http://n2t.net/addgene:241323 ; RRID:Addgene_241323)

  • For your References section:

    Assessing the effect of bovine MSTN variants on pre-mRNA splicing. Gaiani N, Rocha D, Boulling A. Anim Genet. 2026 Feb;57(1):e70073. doi: 10.1002/age.70073. 10.1002/age.70073 PubMed 41614706
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