V5-Apex2-RAP
(Plasmid
#241358)
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PurposeThe V5-Apex2-RAP is a fusion between Apex2 and RAP for recombinant protein production.
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 241358 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepGEX2T
- Backbone size w/o insert (bp) 4948
- Total vector size (bp) 6751
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameRAP
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SpeciesH. sapiens (human)
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Insert Size (bp)1803
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Entrez GeneLRPAP1 (a.k.a. A2MRAP, A2RAP, HBP44, MRAP, MYP23, RAP, alpha-2-MRAP)
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Tag
/ Fusion Protein
- V5-Apex2 (N terminal on insert)
Resource Information
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A portion of this plasmid was derived from a plasmid made bypGEX2T-RAP was from Dr. Dudley Strickland and Apex2 was from Dr. Alice Ting.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
V5-Apex2-RAP was a gift from Jonathan Barasch (Addgene plasmid # 241358 ; http://n2t.net/addgene:241358 ; RRID:Addgene_241358)