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pLVX-IRES-NEO-N-WASP-WH1
(Plasmid #241372)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 241372 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    PLVX-IRES-NEO
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    N-WASP fragment
  • Alt name
    WASL
  • Species
    H. sapiens (human)
  • Mutation
    aa 1-141
  • Entrez Gene
    WASL (a.k.a. N-WASP, NWASP, WASPB)
  • Promoter CMV
  • Tag / Fusion Protein
    • FLAG (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note: This plasmid contains a M197L mutation in NeoR/KanR. It is not known how this mutation affects plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLVX-IRES-NEO-N-WASP-WH1 was a gift from Gaudenz Danuser (Addgene plasmid # 241372 ; http://n2t.net/addgene:241372 ; RRID:Addgene_241372)

  • For your References section:

    Morphological control of merlin-Rac antagonism in proliferation-promoting signaling. Weiss BG, Keth JM, Bhatt K, Doyal M, Hahn KM, Noh J, Isogai T, Danuser G. Sci Signal. 2025 May 20;18(887):eadk0922. doi: 10.1126/scisignal.adk0922. Epub 2025 May 20. 10.1126/scisignal.adk0922 PubMed 40392939
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