pFLAG-CMV4-GAL4 DBD-Aire CTT
(Plasmid
#241399)
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PurposeMammalian gene expression vector for GAL4 DNA binding domain fused to Aire C-terminal tail (aa 491-552)
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 241399 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepFLAG-CMV-4
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGal4-DBD Aire C-terminal tail
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SpeciesM. musculus (mouse)
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MutationTruncated Aire; contains aa 491-552
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Entrez GeneAIRE (a.k.a. AIRE1, APECED, APS1, APSI, PGA1)
- Promoter CMV
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Tag
/ Fusion Protein
- FLAG (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
- 5′ sequencing primer CMV Forward (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pFLAG-CMV4-GAL4 DBD-Aire CTT was a gift from Sun Hur (Addgene plasmid # 241399 ; http://n2t.net/addgene:241399 ; RRID:Addgene_241399)