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pcDNA3.1-NES-CAGEc-ETOc (Npu)
(Plasmid #241409)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 241409 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone size w/o insert (bp) 5328
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NES-CAGEc-ETOc (Npu)
  • Species
    H. sapiens (human), Synthetic
  • Insert Size (bp)
    2601
  • Entrez Gene
    RUNX1T1 (a.k.a. AML1-MTG8, AML1T1, CBFA2T1, CDR, ETO, MTG8, ZMYND2)
  • Promoter CMV
  • Tags / Fusion Proteins
    • 3xFLAG (N terminal on insert)
    • HA (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Engineered NpuC intein (Caged NpuC) fused to the 5'-end of add-on sequence (CFN)-ETO (31-604 aa). NES (MKK1) and FRB domain used to the 5'-end of Caged NpuC.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1-NES-CAGEc-ETOc (Npu) was a gift from Thomas Muir (Addgene plasmid # 241409 ; http://n2t.net/addgene:241409 ; RRID:Addgene_241409)

  • For your References section:

    Distinct phases of cellular signaling revealed by time-resolved protein synthesis. Lee G, Muir TW. bioRxiv [Preprint]. 2023 Jul 10:2023.07.10.548208. doi: 10.1101/2023.07.10.548208. 10.1101/2023.07.10.548208 PubMed 37503273
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