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pLENTICRISPR V2 hygro sgmPOLE_1
(Plasmid #241442)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 241442 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pLENTICRISPR V2 hygro
  • Backbone manufacturer
    Brett Stringer (Addgene #98291)
  • Vector type
    Mammalian Expression, CRISPR
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    sgmPOLE_1
  • gRNA/shRNA sequence
    GGAGCGTCGCAATGGTCCTG
  • Species
    Synthetic
  • Entrez Gene
    POLE (a.k.a. CRCS12, FILS, IMAGEI, POLE1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLENTICRISPR V2 hygro sgmPOLE_1 was a gift from Richard Possemato (Addgene plasmid # 241442 ; http://n2t.net/addgene:241442 ; RRID:Addgene_241442)
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