pLENTICRISPR V2 Hygro sgRELA_4
(Plasmid
#241446)
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PurposeDelete RELA
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 241446 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepLENTICRISPR V2
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Backbone manufacturerFeng Zhang (Addgene #52961)
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Vector typeMammalian Expression, CRISPR
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namesgRELA_4
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gRNA/shRNA sequenceGGGAAGATCTCATCCCCACCG
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SpeciesSynthetic
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Entrez GeneRELA (a.k.a. AIF3BL3, CMCU, NFKB3, p65)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLENTICRISPR V2 Hygro sgRELA_4 was a gift from Richard Possemato (Addgene plasmid # 241446 ; http://n2t.net/addgene:241446 ; RRID:Addgene_241446)