pLENTICRISPR V2 Hygro sgRELB_2
(Plasmid
#241448)
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PurposeDelete RELB
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 241448 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepLENTICRISPR V2
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Backbone manufacturerFeng Zhang (Addgene #52961)
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Vector typeMammalian Expression, CRISPR
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namesgRELB_2
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gRNA/shRNA sequenceGGTGGGGAAAGACTGCACCGA
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SpeciesSynthetic
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Entrez GeneRELB (a.k.a. I-REL, IMD53, IREL, REL-B)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
- 5′ sequencing primer Unknown
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLENTICRISPR V2 Hygro sgRELB_2 was a gift from Richard Possemato (Addgene plasmid # 241448 ; http://n2t.net/addgene:241448 ; RRID:Addgene_241448)