pLENTICRISPR V2-mCherry sgmRELA_2
(Plasmid
#241453)
-
PurposeDelete mouse Rela
-
Depositing Lab
-
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 241453 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
-
Vector backbonepLENTICRISPR V2-mCherry
-
Backbone manufacturerAgata Smogorzewska (Addgene #99154)
-
Vector typeMammalian Expression, CRISPR
-
Selectable markersmCherry
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)NEB Stable
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert namesgmRELA_2
-
gRNA/shRNA sequenceGGTGGGGAAGGACTGCCGGGA
-
SpeciesSynthetic
-
GenBank ID
-
Entrez GeneRELA (a.k.a. AIF3BL3, CMCU, NFKB3, p65)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
- 5′ sequencing primer Unknown
- (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pLENTICRISPR V2-mCherry sgmRELA_2 was a gift from Richard Possemato (Addgene plasmid # 241453 ; http://n2t.net/addgene:241453 ; RRID:Addgene_241453)