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pBHM2673
(Plasmid #241720)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 241720 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pSDMA25
  • Backbone manufacturer
    James Fraser (Addgene Plasmid #67940)
  • Modifications to backbone
    Removed NAT resistance marker from pSDMA25 and replaced with synthesized BSD marker. The BSD coding sequence was codon-optimized for Cryptococcus neoformans and contains an intron.
  • Vector type
    Yeast Expression
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBHM2673 was a gift from Hiten Madhani (Addgene plasmid # 241720 ; http://n2t.net/addgene:241720 ; RRID:Addgene_241720)

  • For your References section:

    CRISPR/Cas9-compatible plasmids enabling seven dominant genetic selection methods for the human fungal pathogen Cryptococcus neoformans. Boucher MJ, Madhani HD. Microbiol Spectr. 2025 Sep 25:e0193525. doi: 10.1128/spectrum.01935-25. 10.1128/spectrum.01935-25 PubMed 40996288
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