pAAV-CAMKII-DrBphP-PCM
(Plasmid
#241775)
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PurposeTo produce AAV9 encoding CAMKII-DrBphP-PCM
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 241775 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 |
Backbone
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Vector backbonepAAV-CAMKII
- Backbone size w/o insert (bp) 3755
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Vector typeAAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameDrBphP-PCM
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SpeciesDeinococcus radiodurans
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Insert Size (bp)1512
- Promoter CAMKII
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NheI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer pBR322ori-F
- 3′ sequencing primer WPRE-R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Expression of DrBphP-PCM driven by the CAMKII promoter. From NCBI reference sequence NC_001264.1.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV-CAMKII-DrBphP-PCM was a gift from Vladislav Verkhusha (Addgene plasmid # 241775 ; http://n2t.net/addgene:241775 ; RRID:Addgene_241775) -
For your References section:
Deep-tissue high-sensitivity multimodal imaging and optogenetic manipulation enabled by biliverdin reductase knockout. Kasatkina LA, Ma C, Sheng H, Lowerison M, Menozzi L, Baloban M, Tang Y, Xu Y, Humayun L, Vu T, Song P, Yao J, Verkhusha VV. Nat Commun. 2025 Jul 14;16(1):6469. doi: 10.1038/s41467-025-61532-4. 10.1038/s41467-025-61532-4 PubMed 40659617