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pAAV-G12-AkaLuc-P2A-Proinsulin
(Plasmid #241776)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 241776 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pAAV-G12
  • Backbone manufacturer
    GeneScript
  • Backbone size w/o insert (bp) 3644
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    AkaLuc-P2A-Proinsulin
  • Species
    M. musculus (mouse), Synthetic
  • Insert Size (bp)
    2086
  • Promoter 12xUAS with TATA-box minimal promoter

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer pBR322ori-F
  • 3′ sequencing primer WPRE-R
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid enables expression of the luciferase reporter AkaLuc and rodent proinsulin, both driven by the G12 promoter. The rodent proinsulin coding sequence was synthesized based on the sequence published in: 10.1016/j.ymthe.2021.09.004.

Akaluc GenBank accession number: LC320664.1.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-G12-AkaLuc-P2A-Proinsulin was a gift from Vladislav Verkhusha (Addgene plasmid # 241776 ; http://n2t.net/addgene:241776 ; RRID:Addgene_241776)

  • For your References section:

    Deep-tissue high-sensitivity multimodal imaging and optogenetic manipulation enabled by biliverdin reductase knockout. Kasatkina LA, Ma C, Sheng H, Lowerison M, Menozzi L, Baloban M, Tang Y, Xu Y, Humayun L, Vu T, Song P, Yao J, Verkhusha VV. Nat Commun. 2025 Jul 14;16(1):6469. doi: 10.1038/s41467-025-61532-4. 10.1038/s41467-025-61532-4 PubMed 40659617
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