pDS_TempRecording
(Plasmid
#241789)
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PurposepBBR1MCS replicative vector for Cas1-Cas2-RT and Spacer for transcriptional recording with a temperature sensor
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 241789 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 |
Backbone
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Vector backbonepBBR1MCS
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Vector typeBacterial Expression, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameFBFP, Cas1-Cas2-RT_and CRISPR Array
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SpeciesDinoroseobacter Shibae
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MutationWT
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDS_TempRecording was a gift from Marc Güell (Addgene plasmid # 241789 ; http://n2t.net/addgene:241789 ; RRID:Addgene_241789) -
For your References section:
Engineered Marine Biofilms for Ocean Environment Monitoring. Nevot G, Pol Cros M, Toloza L, Campama-Sanz N, Artigues-Lleixa M, Aguilera L, Guell M. ACS Synth Biol. 2025 Jun 22. doi: 10.1021/acssynbio.5c00192. 10.1021/acssynbio.5c00192 PubMed 40545707