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pAAV-hSyn-DIO-PGC-1alpha-IRES-mCitrine
(Plasmid #241791)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 241791 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    AAV-hSyn-DIO-hM3D(Gq)-IRES-mCitrine
  • Backbone manufacturer
    Bryan Roth, Addgene plasmid #50454
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PGC-1alpha
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2394
  • Entrez Gene
    Ppargc1a (a.k.a. A830037N07Rik, Gm11133, PGC-1, PPARGC-1-alpha, Pgc-1alpha, Pgc1, Pgco1, Ppargc1)
  • Promoter hSyn
  • Tag / Fusion Protein
    • mCitrine (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI/StuI (unknown if destroyed)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The gene of interest (GOI) was obtained from another lab at UMB

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

We observed a Q339K substitution in the ORF, which was also present in the control plasmid. It is unlikely to affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-hSyn-DIO-PGC-1alpha-IRES-mCitrine was a gift from Mary Kay Lobo (Addgene plasmid # 241791 ; http://n2t.net/addgene:241791 ; RRID:Addgene_241791)

  • For your References section:

    A Role for Peroxisome Proliferator-Activated Receptor Gamma Coactivator-1alpha in Nucleus Accumbens Neuron Subtypes in Cocaine Action. Chandra R, Engeln M, Francis TC, Konkalmatt P, Patel D, Lobo MK. Biol Psychiatry. 2017 Apr 1;81(7):564-572. doi: 10.1016/j.biopsych.2016.10.024. Epub 2016 Oct 28. 10.1016/j.biopsych.2016.10.024 PubMed 27939396
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