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Addgene

pLVX-Fbxw7opt
(Plasmid #241795)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 241795 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pLVX-Tight-Puro
  • Backbone manufacturer
    Clontech
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Fbxw7
  • Species
    H. sapiens (human)
  • GenBank ID
    NM_033632
  • Entrez Gene
    FBXW7 (a.k.a. AGO, CDC4, DEDHIL, FBW6, FBW7, FBX30, FBXO30, FBXW6, SEL-10, SEL10, hAgo, hCdc4)

Cloning Information

  • Cloning method TOPO Cloning
  • 5′ sequencing primer cccGGATCCGCCACCATGAAT
  • 3′ sequencing primer cccGAATTCctACTTGTCGTCGT
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLVX-Fbxw7opt was a gift from Diego Castrillon (Addgene plasmid # 241795 ; http://n2t.net/addgene:241795 ; RRID:Addgene_241795)

  • For your References section:

    Fbxw7 is a driver of uterine carcinosarcoma by promoting epithelial-mesenchymal transition. Cuevas IC, Sahoo SS, Kumar A, Zhang H, Westcott J, Aguilar M, Cortez JD, Sullivan SA, Xing C, Hayes DN, Brekken RA, Bae-Jump VL, Castrillon DH. Proc Natl Acad Sci U S A. 2019 Nov 26. pii: 1911310116. doi: 10.1073/pnas.1911310116. 10.1073/pnas.1911310116 PubMed 31772025
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