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Addgene

pLVX-Pax2
(Plasmid #241796)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 241796 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pLVX-Tight-Puro
  • Backbone manufacturer
    Clontech
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PAX2
  • Species
    H. sapiens (human)
  • GenBank ID
    NM_003987
  • Entrez Gene
    PAX2 (a.k.a. FSGS7, PAPRS, PAX-2)

Cloning Information

  • Cloning method TOPO Cloning
  • 5′ sequencing primer GGGAATTCGTCGACTGGATC
  • 3′ sequencing primer GGGAATTCTTAAACCTTATCGTCGTC
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    OriGene

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2024.12.23.630121 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLVX-Pax2 was a gift from Diego Castrillon (Addgene plasmid # 241796 ; http://n2t.net/addgene:241796 ; RRID:Addgene_241796)

  • For your References section:

    A distinct mechanism of epigenetic reprogramming silences PAX2 and initiates endometrial carcinogenesis. Sahoo SS, Ramanand SG, Cuevas IC, Gao Y, Lee S, Abbas A, Zhang X, Kumar A, Koduru P, Roy S, Broaddus RR, Bae-Jump VL, Gladden AB, Lea J, Lucas E, Xing C, Kobayashi A, Mani RS, Castrillon DH. J Clin Invest. 2025 Aug 15;135(16):e190989. doi: 10.1172/JCI190989. eCollection 2025 Aug 15. 10.1172/JCI190989 PubMed 40829174
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