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pSS22-IRES-NLS-eGFP
(Plasmid #241837)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 241837 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    CIRTS-1: ORF5-TBP6.7-Pin domain-NLS plasmid
  • Vector type
    Donor plasmid

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    eGFP

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The pSS22-IRES-NLS-eGFP plasmid can be restriction digested with BstEII and AvrII, followed by Gibson Assembly to insert GSLinker-mGreenLantern upstream of the IRES. mGreenLantern sequence is available as a supplemental document.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSS22-IRES-NLS-eGFP was a gift from Albert Keung (Addgene plasmid # 241837 ; http://n2t.net/addgene:241837 ; RRID:Addgene_241837)

  • For your References section:

    A human Angelman Syndrome class II pluripotent stem cell line with fluorescent paternal UBE3A reporter. Kelkar GR, Stuppy SR, Sen D, Yagci ZB, Han L, Land L, Hartman JK, Keung AJ. Front Cell Dev Biol. 2025; 13:1665693. https://doi.org/10.3389/fcell.2025.1665693 10.3389/fcell.2025.1665693
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