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Addgene

pDS48
(Plasmid #241839)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 241839 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pX330-U6-Chimeric\_BB-Cb-hSpCas9
  • Vector type
    Mammalian Expression, AAV, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cas9-gRNA
  • gRNA/shRNA sequence
    AGGCCATCACGTATGCCAA
  • Entrez Gene
    UBE3A (a.k.a. ANCR, AS, E6-AP, EPVE6AP, HPVE6A, PIX1)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDS48 was a gift from Albert Keung (Addgene plasmid # 241839 ; http://n2t.net/addgene:241839 ; RRID:Addgene_241839)

  • For your References section:

    A human Angelman Syndrome class II pluripotent stem cell line with fluorescent paternal UBE3A reporter. Kelkar GR, Stuppy SR, Sen D, Yagci ZB, Han L, Land L, Hartman JK, Keung AJ. Front Cell Dev Biol. 2025; 13:1665693. https://doi.org/10.3389/fcell.2025.1665693 10.3389/fcell.2025.1665693
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