pPB-p53RE(CDKN1A)-CMVmin-mScarlet-I-NLSx3-AU1-PGKpuro
(Plasmid #241845)

• Purpose: Expresses p53 transcription fluorescence reporter in mammalian cells.
• Depositing Lab: Kazuhiro Aoki
• Publication: Tsuruoka et al Cell Struct Funct. 2025 Jun 12. doi: 10.1247/csf.25017.

Backbone

• Vector backbone: pPB
• Backbone size w/o insert (bp): 6226
• Total vector size (bp): 7389
• Modifications to backbone: The backbone contains Insulator-PGK promoter-pac gene-SV40 late polyA cassette for the selection marker.
• Vector type: Mammalian Expression
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CDKN1A promoter
• Species: H. sapiens (human)
• Mutation: CDKN1A promoter (1015 bp) + CMV minimal promoter (125 bp)
• Entrez Gene: CDKN1A (a.k.a. CAP20, CDKN1, CIP1, MDA-6, P21, SDI1, WAF1, p21CIP1)
• Promoter: n/a
• Tag / Fusion Protein:
  • mScarlet-I-3xNLS (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: NotI (unknown if destroyed)
• 5′ sequencing primer: n/a
• 3′ sequencing primer: n/a

Resource Information

• Supplemental Documents:
  • ppb-p53recdkn1a-cmvmin-mscarlet-i-nlsx3-au1-pgkpuro.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • piggyBac Limited Use Label License
• Industry Terms:
  • Not Available to Industry