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pPBbsr2-NLSx3-miRFP703-dSal-p53NT(1-97)-5SD-lin-hCRY2
(Plasmid #241846)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 241846 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pPB
  • Backbone size w/o insert (bp) 6774
  • Total vector size (bp) 9549
  • Vector type
    Mammalian Expression
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    p53
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    291
  • Mutation
    N-terminus (1-97 aa) containing five phosphorylation-mimetic mutations to enhance the interaction between p53 and the transcriptional coactivator
  • Entrez Gene
    TP53 (a.k.a. BCC7, BMFS5, LFS1, P53, TRP53)
  • Promoter CAGGS promoter
  • Tags / Fusion Proteins
    • miRFP703 (N terminal on insert)
    • CRY2 (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer n/a
  • 3′ sequencing primer n/a
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPBbsr2-NLSx3-miRFP703-dSal-p53NT(1-97)-5SD-lin-hCRY2 was a gift from Kazuhiro Aoki (Addgene plasmid # 241846 ; http://n2t.net/addgene:241846 ; RRID:Addgene_241846)

  • For your References section:

    Opto-p53: A Light-Controllable Activation of p53 Signaling Pathway. Tsuruoka T, Goto Y, Aoki K. Cell Struct Funct. 2025 Jun 12. doi: 10.1247/csf.25017. 10.1247/csf.25017 PubMed 40500178
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