pPBneo-CIBN-lin-p53CT(98-393)-R273H-mNeonGreen-NLSx3
(Plasmid
#241849)
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PurposeExpresses a negative control of the localizer of Opto-p53 in mammalian cells.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 241849 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 |
Backbone
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Vector backbonepPB
- Backbone size w/o insert (bp) 7163
- Total vector size (bp) 9374
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namep53
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SpeciesH. sapiens (human)
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Insert Size (bp)888
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Mutationp53 C-terminus (97-393 aa) containing R273H mutation, which abolishes DNA binding.
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Entrez GeneTP53 (a.k.a. BCC7, BMFS5, LFS1, P53, TRP53)
- Promoter CAGGS promoter
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Tags
/ Fusion Proteins
- CIBN (N terminal on insert)
- mNeonGreen (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer n/a
- 3′ sequencing primer n/a (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pPBneo-CIBN-lin-p53CT(98-393)-R273H-mNeonGreen-NLSx3 was a gift from Kazuhiro Aoki (Addgene plasmid # 241849 ; http://n2t.net/addgene:241849 ; RRID:Addgene_241849) -
For your References section:
Opto-p53: A Light-Controllable Activation of p53 Signaling Pathway. Tsuruoka T, Goto Y, Aoki K. Cell Struct Funct. 2025 Jun 12. doi: 10.1247/csf.25017. 10.1247/csf.25017 PubMed 40500178