pMAL-sfGFP-mCRISPRed
(Plasmid
#241869)
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PurposeExpresses a fusion protein of sfGFP and mCRISPRed with N-terminal maltose binding protein in bacteria
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 241869 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepMAL
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Backbone manufacturerNew England Biolabs
- Total vector size (bp) 7149
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameSuperfolder GFP-mCRISPRed fusion protein
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SpeciesSynthetic
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made bymCRISPRed synthesized based on doi: 10.1016/j.chembiol.2020.02.004 sfGFP synthesized based on doi: 10.1038/nbt0906-1170d
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMAL-sfGFP-mCRISPRed was a gift from Ian Thornell (Addgene plasmid # 241869 ; http://n2t.net/addgene:241869 ; RRID:Addgene_241869) -
For your References section:
The pH-dependent photophysical and spectral properties of pH-sensing green fluorescent proteins. Thornell IM. Physiol Rep. 2025 Oct;13(20):e70625. doi: 10.14814/phy2.70625. 10.14814/phy2.70625 PubMed 41108577